منابع مشابه
Human cathepsin S: chromosomal localization, gene structure, and tissue distribution.
The human lysosomal cysteine proteinases, cathepsins H, L, and B, have been mapped to chromosomes 15, 9, and 8, respectively, and the genomic structures of cathepsins L and B have been determined. We report here the chromosomal localization and partial gene structure for a recently sequenced human cysteine proteinase, cathepsin S. A 20-kilobase pair genomic clone of the human cathepsin S gene w...
متن کاملCharacterization of Recombinant Rat Cathepsin
The cysteine proteinase rat cathepsin B was expressed in yeast in an active form and was found to be heterogeneously glycosylated at the consensus sequence for N-linked oligosaccharide substitution. Purified enzyme fractions containing the highest levels of glycosylation were shown to have reduced activity. A glycosylation minus mutant constructed by site-directed mutagenesis (by changing the S...
متن کاملFormation Kinetics of Structure H Gas Hydrate
This paper investigates the kinetics of structure H (sH) formation kinetics above and below the structure I (sI) formation equilibrium curve at temperatures of between 2°C and 6°C. Methane was used as a help gas and methylcyclohexane (MCH) was used as sH former. It was concluded that in the points above the sI formation equilibrium curve, at the first, the sI forms, and then converts to sH be...
متن کاملCrystallization of recombinant rat cathepsin B.
A glycosylation-minus mutant of rat cathepsin B expressed in yeast has been purified and crystallized. X-ray diffraction data have been collected and molecular replacement for solving the structure is in progress. The space group for the recombinant rat cathepsin B was determined to be P2(1) with unit cell dimensions alpha = 62.2 A, b = 90.19 A, c = 47.07 A, and beta = 97.43 degrees. A unit cel...
متن کاملGene Targeting of the Cysteine Peptidase Cathepsin H Impairs Lung Surfactant in Mice
BACKGROUND The 11 human cysteine cathepsins are proteases mainly located in the endolysosomal compartment of all cells and within the exocytosis pathways of some secretory cell types. Cathepsin H (Ctsh) has amino- and endopeptidase activities. In vitro studies have demonstrated Ctsh involvement in the processing and secretion of the pulmonary surfactant protein B (SP-B). Furthermore, Ctsh is hi...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)80939-1